Human COMP Quantikine ELISA Kit

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Human COMP Quantikine ELISA Kit

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Human COMP Quantikine ELISA Kit Summary

货号：DCMP0 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.036 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human COMP

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human COMP immunoassay is a 4.5 hour solid phase ELISA designed to measure human COMP in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human COMP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human COMP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring COMP.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	0.9	2.92	6.11	1.05	3.07	6.26
Standard Deviation	0.028	0.112	0.225	0.045	0.146	0.3
CV%	2.1	3.8	3.7	4.3	4.8	4.8

Recovery

The recovery of COMP spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	103	95-111
Cell Lysates (n=1)	102	98-108
EDTA Plasma (n=4)	103	86-114
Heparin Plasma (n=4)	99	88-112
Serum (n=4)	102	86-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human COMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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