Human Galectin-1 Quantikine ELISA Kit

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Human Galectin-1 Quantikine ELISA Kit

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Human Galectin-1 Quantikine ELISA Kit Summary

货号货号：DGAL10

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL), Human Milk (50 uL)

Sensitivity

0.129 ng/mL

Assay Range

0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk)

Specificity

Natural and recombinant human Galectin-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Galectin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Galectin-1 in cell culture supernates, serum, plasma, saliva, and human milk. It contains E. coli-expressed recombinant human Galectin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Galectin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Galectin-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	1.96	4.66	8.51	2.07	5.02	9.37
Standard Deviation	0.139	0.408	0.483	0.197	0.444	0.701
CV%	7.1	8.8	5.7	9.5	8.8	7.5

Recovery

The recovery of Galectin-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	93-115
EDTA Plasma (n=4)	96	87-102
Heparin Plasma (n=4)	93	80-102
Serum (n=4)	96	82-104

Linearity

To assess the linearity of the assay, samples containing high concentrations of Galectin-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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