Human LIGHT/TNFSF14 Quantikine ELISA Kit

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Human LIGHT/TNFSF14 Quantikine ELISA Kit

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Human LIGHT/TNFSF14 Quantikine ELISA Kit Summary

货号：DLIT00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (25 uL)

Sensitivity

16.5 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)

Specificity

Natural and recombinant human LIGHT

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human LIGHT Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human LIGHT in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human LIGHT and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human LIGHT showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human LIGHT.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	350	712	1388	387	754	1469
Standard Deviation	12	31.7	63.3	19.6	31.8	63.4
CV%	3.4	4.5	4.6	5.1	4.2	4.3

Cell Culture Supernates, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	308	626	1181	318	616	1177
Standard Deviation	11.7	25.3	34.6	27	44.2	81.8
CV%	3.8	4	2.9	8.5	7.2	6.9

Recovery

The recovery of LIGHT spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	105	100-113
EDTA Plasma (n=5)	106	91-114
Heparin Plasma (n=5)	101	89-115
Serum (n=5)	102	85-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of LIGHT were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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