Human ACE Quantikine ELISA Kit

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Human ACE Quantikine ELISA Kit

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Human ACE Quantikine ELISA Kit Summary

货号：DACE00 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL), Saliva (25 uL)

Sensitivity

0.051 ng/mL

Assay Range

0.8 - 50 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva)

Specificity

Natural and recombinant human ACE. In addition to soluble ACE, this kit recognizes membrane-bound forms from lysates of cultured cells and supernates.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human ACE Immunoassay is a 4.5 hour solid phase ELISA designed to measure ACE in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed ectodomain of recombinant human sACE, and antibodies raised against the recombinant factor. Natural human ACE showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human ACE.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	3.6	10.7	22.1	3.44	10.3	21.8
Standard Deviation	0.08	0.3	0.95	0.21	0.4	0.87
CV%	2.2	2.8	4.3	6.1	3.9	4

Serum, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	3.57	10.9	22.5	3.77	11.7	24.4
Standard Deviation	0.12	0.43	0.9	0.29	0.6	1.2
CV%	3.4	3.9	4	7.7	5.1	4.9

Recovery

The recovery of ACE spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	98	89-106
Heparin Plasma (n=4)	100	94-106
Serum (n=4)	99	91-106

Linearity

To assess the linearity of the assay, samples containing high concentrations of ACE were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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