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HIV-1 Gag p24 Quantikine ELISA Kit
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HIV-1 Gag p24 Quantikine ELISA Kit Summary
货号：DHP240
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
3.25 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
HIV-1 Gag p24.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 45.7 | 135 | 280 | 43 | 132 | 285 |
| Standard Deviation | 1.41 | 4.86 | 8.1 | 1.93 | 4.61 | 11.2 |
| CV% | 3.1 | 3.6 | 2.9 | 4.5 | 3.5 | 3.9 |
Recovery
The recovery of HIV-1 Gag p24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 93-105 |
| EDTA Plasma (n=4) | 74 | 64-82 |
| EDTA Plasma (ICD Treated) (n=4) | 92 | 85-100 |
| Heparin Plasma (n=4) | 72 | 63-78 |
| Heparin Plasma (ICD Treated) (n=4) | 88 | 69-94 |
| Serum (n=4) | 79 | 73-85 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of HIV-1 Gag p24 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
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