Human SPARC Quantikine ELISA Kit

返回产品中心

Human SPARC Quantikine ELISA Kit

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

Human SPARC Quantikine ELISA Kit Summary

货号：DSP00 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 uL), Serum (10 uL), Platelet-poor EDTA Plasma (20 uL), Platelet-poor Heparin Plasma (20 uL), Human Milk (50 uL)

Sensitivity

0.269 ng/mL

Assay Range

1.6 - 50 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Human Milk)

Specificity

Natural and recombinant human SPARC

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human SPARC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SPARC in cell culture supernates, serum, plasma, and human milk. It contains NS0-expressed recombinant human SPARC and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SPARC showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human SPARC.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	7.34	14.3	29.9	6.65	13.5	28.3
Standard Deviation	0.129	0.282	0.733	0.541	0.989	2.4
CV%	1.8	2	2.5	8.1	7.3	8.5

Recovery

The recovery of SPARC spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	86-111
Platelet-poor EDTA Plasma (n=4)	104	93-111
Platelet-poor Heparin Plasma (n=4)	91	82-100

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SPARC were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

HIV-1 Gag p24 Quantikine ELISA Kit