Human Cyr61/CCN1 Quantikine ELISA Kit

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Human Cyr61/CCN1 Quantikine ELISA Kit

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Human Cyr61/CCN1 Quantikine ELISA Kit Summary

货号：DCYR10 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Saliva (50 uL), Human Milk (10 uL)

Sensitivity

3.8 pg/mL

Assay Range

39.1 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Saliva, Human Milk)

Specificity

Natural and recombinant human Cyr61

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Cyr61/CCN1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Cyr61 in cell culture supernates, serum, plasma, saliva, and human milk. It contains CHO cell-expressed recombinant human Cyr61 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Cyr61 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Cyr61.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Saliva, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	256	721	1442	249	712	1445
Standard Deviation	5.94	14.2	33.5	15.9	35	73.3
CV%	2.3	2	2.3	6.4	4.9	5.1

Recovery

The recovery of Cyr61 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	107	98-117
EDTA Plasma (n=4)	98	91-104
Serum (n=4)	101	95-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Cyr61 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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