Human Uteroglobin Quantikine ELISA Kit

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Human Uteroglobin Quantikine ELISA Kit

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Human Uteroglobin Quantikine ELISA Kit Summary

货号：DUGB00 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL), Human Milk (50 uL)

Sensitivity

0.217 ng/mL

Assay Range

0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

Specificity

Natural and recombinant human Uteroglobin

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Uteroglobin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Uteroglobin in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Uteroglobin and has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human Uteroglobin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Uteroglobin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Saliva, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	5.1	15.4	31.3	5.2	15.4	29.9
Standard Deviation	0.29	0.34	1.04	0.31	0.72	2.25
CV%	5.7	2.2	3.3	6	4.7	7.5

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	5.2	15.8	31.7	5	14.7	28.8
Standard Deviation	0.21	0.49	1.16	0.28	0.92	2.09
CV%	4	3.1	3.7	5.6	6.3	7.3

Recovery

The recovery of Uteroglobin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	99-109
EDTA Plasma (n=4)	95	85-107
Heparin Plasma (n=4)	95	86-109
Serum (n=4)	95	85-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Uteroglobin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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