The Quantikine Mouse ST2/IL-33 R Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse ST2 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse ST2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse ST2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse ST2.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

The recovery of mouse ST2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

To assess the linearity of the assay, samples containing high concentrations of mouse ST2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.