Mouse LIX Quantikine ELISA Kit

返回产品中心

Mouse LIX Quantikine ELISA Kit

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

Mouse LIX Quantikine ELISA Kit Summary

货号：MX000 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (13 uL), Platelet-poor Heparin Plasma (13 uL), Urine (13 uL)

Sensitivity

1.74 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)

Specificity

Natural and recombinant mouse LIX

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse CXCL5/LIX Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LIX levels in cell culture supernates, tissue lysates, serum, platelet-poor plasma, and urine. It contains E. coli-expressed recombinant mouse LIX and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse LIX accurately. Results obtained using natural mouse LIX showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse LIX.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	42.7	133	407	41.8	120	393
Standard Deviation	1.72	5.93	10.9	2.81	4.64	20.3
CV%	4	4.5	2.7	6.7	3.9	5.2

Recovery

The recovery of mouse LIX spiked to three levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=4)	114	105-120
Platelet-poor EDTA Plasma (n=4)	100	92-109
Platelet-poor Heparin Plasma (n=4)	91	85-99
Serum (n=4)	87	82-94
Tissue Homogenates (n=4)	100	87-113
Urine (n=4)	91	84-96

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse LIX were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Mouse ST2/IL-33 R Quantikine ELISA Kit