Human PDGF-DD Quantikine ELISA Kit

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Human PDGF-DD Quantikine ELISA Kit

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Human PDGF-DD Quantikine ELISA Kit Summary

货号：DDD00 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL), Saliva (50 uL), Human Milk (50 uL)

Sensitivity

3.51 pg/mL

Assay Range

31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Human Milk)

Specificity

Natural and recombinant human PDGF-DD

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human PDGF-DD Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PDGF-DD in cell culture supernates, serum, plasma, saliva, and human milk. It contains NS0-expressed recombinant human PDGF-DD and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-DD showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PDGF-DD.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	176	510	986	198	559	1095
Standard Deviation	3.69	11.9	17.2	16	38.5	55.3
CV%	2.1	2.3	1.7	8.1	6.9	5.1

Recovery

The recovery of PDGF-DD spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	83-112
Platelet-poor EDTA Plasma (n=4)	93	83-109
Platelet-poor Heparin Plasma (n=4)	94	84-113
Serum (n=4)	101	86-112

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PDGF-DD were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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