Human EGFR Quantikine ELISA Kit

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Human EGFR Quantikine ELISA Kit

Human EGFR Quantikine ELISA Kit Summary

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货号：DEGFR0 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Human Milk (50 uL)

Sensitivity

0.036 ng/mL

Assay Range

0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk)

Specificity

Natural and recombinant human EGF R

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human EGF R immunoassay is a 4.5 hour solid phase ELISA designed to measure EGF R in cell culture supernates, serum, plasma, and human milk. It contains NS0-expressed recombinant human EGF R and has been shown to accurately quantitate the recombinant factor. Results obtained using natural EGF R showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human EGF R.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	1.65	5.08	10.6	1.63	5	10
Standard Deviation	0.094	0.17	0.507	0.144	0.416	0.816
CV%	5.7	3.3	4.8	8.8	8.3	8.2

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	1.69	5.33	11.2	1.7	5.21	10.4
Standard Deviation	0.072	0.199	0.616	0.161	0.522	1.03
CV%	4.3	3.7	5.5	9.4	10	10

Recovery

The recovery of EGF R spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	86-106
EDTA Plasma (n=4)	99	91-108
Heparin Plasma (n=4)	101	92-111
Serum (n=4)	101	89-117

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of EGF R were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Product Summary

Precision

Recovery

Linearity

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