Human MBL Quantikine ELISA Kit

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Human MBL Quantikine ELISA Kit

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Human MBL Quantikine ELISA Kit Summary

货号：DMBL00 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.029 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Oligomeric forms of natural and recombinant human MBL

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MBL immunoassay is a 4.5 hour solid phase ELISA designed to measure human MBL in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human MBL and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MBL showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of naturally occurring human MBL.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	0.91	2.05	4.06	1.02	2.06	4.14
Standard Deviation	0.03	0.07	0.22	0.08	0.14	0.29
CV%	3.3	3.4	5.4	7.8	6.8	7

Recovery

The recovery of MBL spiked to various levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	113	106-117

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of natural MBL were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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