Human IL-19 Quantikine ELISA Kit

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Human IL-19 Quantikine ELISA Kit

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Human IL-19 Quantikine ELISA Kit Summary

货号：D1900 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL), Human Milk (50 uL)

Sensitivity

12.2 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk)

Specificity

Natural and recombinant human IL-19

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-19 immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-19 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human IL-19 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-19 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IL-19.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	292	805	1586	269	739	1478
Standard Deviation	10.3	19	41.8	22	56.8	103
CV%	3.5	2.4	2.6	8.2	7.7	7

Recovery

The recovery of human IL-19 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	93-104
EDTA Plasma (n=4)	94	85-102
Heparin Plasma (n=4)	93	87-99
Serum (n=4)	93	87-97

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-19 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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