Human Chemerin Quantikine ELISA Summary

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Human Chemerin Quantikine ELISA Summary

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Human Chemerin Quantikine ELISA Summary

Catalog #	Availability	Size / Price
DCHM00	Contact your distributor for availability.	1 Kit / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

7.8 pg/mL

Assay Range

78.0 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Chemerin

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Chemerin Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Chemerin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Chemerin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Chemerin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Chemerin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	513	1558	3016	515	1592	3147
Standard Deviation	23.3	66.5	84.6	40.5	121	200
CV%	4.5	4.3	2.8	7.9	7.6	6.4

Recovery

The recovery of Chemerin spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=8)	104	94-111

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Chemerin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Chemerin ELISA Linearity

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