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Human AgRP Quantikine ELISA Kit

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Human AgRP Quantikine ELISA Kit

Catalog #	Availability	Size / Price
DAGR00	Contact your distributor for availability.	1 Kit / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (25 uL)

Sensitivity

2.68 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human AgRP

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human AgRP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human AgRP in cell culture supernates, plasma, and urine. It contains Sf 21-expressed recombinant human AgRP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human AgRP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human AgRP.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	56.6	144	262	45.1	130	246
Standard Deviation	3.14	4.86	7.82	4.66	10	18.1
CV%	5.5	3.4	3	10.3	7.7	7.4

Cell Culture Supernates, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	54.3	143	257	43.7	126	238
Standard Deviation	2.64	3.88	5.69	3.91	8.24	16.3
CV%	4.9	2.7	2.2	8.9	6.5	6.8

Recovery

The recovery of AgRP spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	106	95-115
EDTA Plasma (n=4)	96	89-106
Heparin Plasma (n=4)	92	84-103

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of AgRP were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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