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Human ADAM12 Quantikine ELISA Kit

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Human ADAM12 Quantikine ELISA Kit

Catalog #	Availability	Size / Price
DAD120	Contact your distributor for availability.	1 Kit / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (25 uL), Heparin Plasma (25 uL), Urine (50 uL)

Sensitivity

0.076 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Urine)

Specificity

Natural and recombinant human ADAM12. This kit recognizes pro-ADAM12, mature ADAM12, and ADAM12/TIMP-3 complex.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human ADAM12 immunoassay is a 4.5 hour solid phase ELISA designed to measure human ADAM12 in cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human ADAM12 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural ADAM12 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring ADAM12.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	1.39	3.57	6.84	1.43	3.62	6.74
Standard Deviation	0.057	0.131	0.235	0.079	0.145	0.299
CV%	4.1	3.7	3.4	5.5	4	4.4

Recovery

The recovery of human ADAM12 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=8)	98	88-105
Heparin Plasma (n=4)	97	92-106
Serum (n=4)	98	89-106
Urine (n=4)	99	91-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ADAM12 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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