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Human PSG1 Quantikine ELISA Kit

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Human PSG1 Quantikine ELISA Kit

Catalog #	Availability	Size / Price
DPSG10	Contact your distributor for availability.	1 Kit / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 ng/mL), Serum (50 ng/mL), EDTA Plasma (50 ng/mL), Heparin Plasma (50 ng/mL)

Sensitivity

0.48 ng/mL

Assay Range

3.1 - 200 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human PSG-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human PSG-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PSG-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human PSG-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PSG-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PSG-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	18.7	59.4	118	20	62.4	121
Standard Deviation	0.36	0.79	1.7	1.2	2.4	4.9
CV%	1.9	1.3	1.4	6	3.8	4

Recovery

The recovery of PSG-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	100	83-118
EDTA Plasma (n=4)	92	84-108
Heparin Plasma (n=4)	90	83-99
Serum (n=4)	91	81-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PSG-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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