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Human Kallikrein 5 Quantikine ELISA Kit

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Human Kallikrein 5 Quantikine ELISA Kit

Catalog #	Availability	Size / Price
DKK500	Contact your distributor for availability.	1 Kit / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (13 uL), Human Milk (13 uL)

Sensitivity

30 pg/mL

Assay Range

78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk)

Specificity

Natural and recombinant human KLK5

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human KLK5 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human KLK5 in cell culture supernates, serum, plasma, saliva, and human milk. It contains NS0-expressed recombinant human KLK5 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human KLK5 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human KLK5.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	510	1685	3407	577	1941	3852
Standard Deviation	35.8	57.2	75.4	63.6	161	278
CV%	7	3.4	2.2	11	8.3	7.2

Recovery

The recovery of KLK5 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	97	91-109
EDTA Plasma (n=4)	90	82-95
Heparin Plasma (n=4)	91	86-103
Serum (n=4)	93	85-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of KLK5 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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