返回产品中心

Human MIA Quantikine ELISA Kit

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

Human MIA Quantikine ELISA Kit

Catalog #	Availability	Size / Price
DMIA00	Contact your distributor for availability.	1 Kit / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Saliva (50 uL)

Sensitivity

8.46 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)

Specificity

Natural and recombinant human MIA

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MIA Immunoassay is a 4.5 hour solid-phase ELISA designed to measure MIA in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant Human MIA and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MIA. Results obtained using natural human MIA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIA.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	158	315	622	150	308	627
Standard Deviation	5.5	10.9	17.8	7.02	15.7	37.8
CV%	3.5	3.5	2.9	4.7	5.1	6

Recovery

The recovery of MIA spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	103	101-107
EDTA Plasma (n=4)	98	85-115
Heparin Plasma (n=4)	99	85-113
Saliva (n=4)	102	86-110
Serum (n=4)	101	91-118

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MIA were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Human Kallikrein 5 Quantikine ELISA Kit