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Mouse IL-6 R alpha Quantikine ELISA Kit

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Mouse IL-6 R alpha Quantikine ELISA Kit

Catalog #	Availability	Size / Price
MR600	Contact your distributor for availability.	1 Kit / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.07 ng/mL

Assay Range

0.2 - 12 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)

Specificity

Natural and recombinant mouse IL-6 R alpha.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-6 R alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-6 R alpha levels in cell culture supernates, serum, and heparin plasma. It contains NS0-expressed recombinant mouse IL-6 R alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-6 R alpha accurately. Results obtained using natural mouse IL-6 R alpha showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6 R alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	0.456	1.07	2.94	0.474	1.08	2.82
Standard Deviation	0.036	0.039	0.148	0.06	0.08	0.2
CV%	7.9	3.6	5	12.7	7.4	7.1

Recovery

The recovery of mouse IL-6 R alpha spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	90-110
Heparin Plasma (n=4)	99	92-106
Serum (n=4)	102	93-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-6 R alpha were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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