Human FOLR1 Quantikine ELISA Kit

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Human FOLR1 Quantikine ELISA Kit

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Human FOLR1 Quantikine ELISA Kit

Catalog #	Availability	Size / Price
DFLR10	Contact your distributor for availability.	1 Kit / Please Inquir

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (10 uL), Urine (10 uL), Human Milk (10 uL)

Sensitivity

14.9 pg/mL

Assay Range

46.9 - 3,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

Specificity

Natural and recombinant human FOLR1. This assay recognizes recombinant human FOLR1 in the presence of folic acid.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human FOLR1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FOLR1 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains CHO cell-expressed recombinant human FOLR1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FOLR1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FOLR1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	377	1024	1922	390	1050	1973
Standard Deviation	9.28	19.8	29.5	20.3	64	135
CV%	2.5	1.9	1.5	5.2	6.1	6.9

Recovery

The recovery of FOLR1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	94-107
EDTA Plasma (n=4)	95	84-106
Heparin Plasma (n=4)	96	82-105
Serum (n=4)	96	84-107

Linearity

To assess the linearity of the assay, samples containing high concentrations of FOLR1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Product Summary

Precision

Recovery

Linearity

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