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Human Attractin Quantikine ELISA Kit

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Human Attractin Quantikine ELISA Kit

Catalog #	Availability	Size / Price
DATRN0	Contact your distributor for availability.	1 Kit / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL)

Sensitivity

0.217 ng/mL

Assay Range

0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)

Specificity

Natural and recombinant human Attractin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Attractin Immunoassay is a 4.5 hour solid phase ELISA designed to measure Attractin levels in cell culture supernates, serum, plasma, saliva, and urine. It contains CHO cell-expressed recombinant human Attractin and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Attractin showed linear curves that were parallel to the standard curves obtained using the Quantikine Human Attractin Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human Attractin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	4.16	12.7	26.2	4.61	13.7	27.5
Standard Deviation	0.132	0.324	0.893	0.498	1.1	1.54
CV%	3.2	2.6	3.4	10.8	8	5.6

Recovery

The recovery of human Attractin spiked to levels throughout the range of the assay was evaluated

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	91	87-97

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Attractin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Axl Quantikine ELISA Kit