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Human Calbindin D Quantikine ELISA Kit

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Human Calbindin D Quantikine ELISA Kit

Catalog #	Availability	Size / Price
DCALD0	Contact your distributor for availability.	1 Kit / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Tissue Lysates (10 uL), Urine (25 uL)

Sensitivity

0.015 ng/mL

Assay Range

0.1 - 5 ng/mL

Specificity

Natural and recombinant human Calbindin D.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Calbindin D Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Calbindin D in tissue lysates and urine. It contains E. coli-expressed recombinant human Calbindin D and antibodies raised against the recombinant factor. Natural human Calbindin D showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural human Calbindin D.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Tissue Lysates, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	0.511	1.65	2.84	0.548	1.57	2.96
Standard Deviation	0.026	0.04	0.072	0.038	0.075	0.15
CV%	5.1	2.4	2.5	6.9	4.8	5.1

Recovery

The recovery of human Calbindin D spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Urine (n=4)	93	89-98

Linearity

To assess the linearity of the assay, samples containing high concentrations of human Calbindin D were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

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