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Mouse Progranulin Quantikine ELISA Kit

Mouse Progranulin Quantikine ELISA Kit

Catalog #	Availability	Size / Price
MPGRN0	Contact your distributor for availability.	1 Kit / Please Inquire

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.071 ng/mL

Assay Range

0.6 - 20 ng/mL (Cell Culture Supernates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse Progranulin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine® Mouse Progranulin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Progranulin in cell culture supernates, tissue culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse Progranulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Progranulin showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Progranulin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	1.75	2.94	7.28	1.69	2.89	7.53
Standard Deviation	0.05	0.067	0.16	0.092	0.137	0.336
CV%	2.9	2.3	2.2	5.4	4.7	4.5

Recovery

The recovery of mouse Progranulin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Culture Media (n=4)	98	94-104

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse Progranulin were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

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