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Human DLL1 Quantikine ELISA Kit

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Human DLL1 Quantikine ELISA Kit

Catalog #	Availability	Size / Price
DLL10	Contact your distributor for availability.	1 Kit / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)

Sensitivity

17.4 pg/mL

Assay Range

78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk)

Specificity

Natural and recombinant human DLL10

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human DLL1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DLL1 in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human DLL1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human DLL1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human DLL1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	638	1468	2934	592	1340	2714
Standard Deviation	16	38.1	76.1	42.4	85.7	189
CV%	2.5	2.6	2.6	7.2	6.4	7

Recovery

The recovery of DLL1 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	96-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of DLL1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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