Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit

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Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit

Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit Summary

Catalog Size / Price
MJE00 1 Kit (2 Plates) / Please Inquire
SMJE00 1 Pack (6 Plates) / Please Inquire

PMJE00 1 Pack (50 Plates) / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 uL), Serum (25 uL)

Sensitivity

2 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum)

Specificity

Natural and recombinant mouse and rat MCP-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat CCL2/MCP-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MCP-1 in mouse or rat cell culture supernates and serum. It contains E. coli-expressed mouse MCP-1 and antibodies raised against the recombinant factor. This Immunoassay has been shown to accurately quantitate recombinant mouse MCP-1. Results obtained using natural mouse or rat MCP-1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural MCP-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	47.1	311	768	43.6	310	783
Standard Deviation	3.9	15.8	40.6	3.2	14.3	42.8
CV%	8.3	5.1	5.3	7.3	4.6	5.5

Recovery

The recovery of MCP-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Mouse Cell Culture Supernates (n=6)	104	91-119
Mouse Serum (n=9)	99	89-113
Rat Cell Culture Supernates (n=4)	109	103-115
Rat Serum (n=4)	109	103-116

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