Mouse IL-10 Quantikine ELISA Kit

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Mouse IL-10 Quantikine ELISA Kit

Mouse IL-10 Quantikine ELISA Kit Summary

Catalog Size / Price
M1000B 1 Kit (2 Plates) / Please Inquire
SM1000B 1 Pack (6 Plates) / Please Inquire

PM1000B 1 Pack (50 Plates) / Please Inquire

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

5.22 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse IL-10

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-10 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-10 accurately. Results obtained using natural mouse IL-10 showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-10.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to assess inter-assay precision over two lots of kit components. Assays were performed by at least three technicians

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	76.3	123	815	71.4	115	809
Standard Deviation	2.56	2.85	17	5.39	7.64	55.5
CV%	3.4	2.3	2.1	7.5	6.6	6.9

Recovery

The recovery of IL-10 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=4)	99	95-103
EDTA Plasma (n=4)	91	86-99
Heparin Plasma (n=4)	89	82-98
Serum (n=4)	89	85-96

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