Human MMP-9 Quantikine ELISA Kit

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Human MMP-9 Quantikine ELISA Kit

Human MMP-9 Quantikine ELISA Kit Summary

Catalog # Availability Size
DMP900 1 Kit
SMP900 1 Pack (6 Plates)
PDMP900 1 Pack (50 Plates)

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 uL), Serum (10 uL), Platelet-poor Heparin Plasma (10 uL), Saliva (10 uL), Urine (100 uL)

Sensitivity

0.156 ng/mL

Assay Range

0.3 - 20 ng/mL (Cell Culture Supernates, Serum, Platelet-poor Heparin Plasma, Saliva, Urine)

Specificity

Natural and recombinant human 92 kDa Pro-MMP-9 and the 82 kDa active MMP-9

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human MMP-9 Immunoassay is a 3.5 hour solid phase ELISA designed to measure MMP-9 (92 kDa pro- and 82 kDa active forms but not the 65 kDa form) in cell culture supernates, saliva, serum, plasma, and urine. It is calibrated with CHO cell-expressed recombinant human pro-MMP-9 and the antibodies were raised against the recombinant factor. Natural human MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative mass values of natural human MMP-9.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Platelet-poor Heparin Plasma, Saliva, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	0.833	2.04	11	0.972	2.35	12.2
Standard Deviation	0.017	0.039	0.316	0.077	0.184	0.845
CV%	2	1.9	2.9	7.9	7.8	6.9

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