Mouse IL-17 Quantikine ELISA Kit

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Mouse IL-17 Quantikine ELISA Kit

Mouse IL-17 Quantikine ELISA Kit Summary

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货号：M1700

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

5 pg/mL

Assay Range

10.9 - 700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse IL-17

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-17 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-17 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-17 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse IL-17 accurately. Results obtained using natural mouse IL-17 showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-17.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	41.9	215	407	43.1	220	411
Standard Deviation	2.1	6.3	8.1	2.2	11.2	18.5
CV%	5	2.9	2	5.1	5.1	4.5

Recovery

The recovery of mouse IL-17 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	101	92-118
EDTA Plasma (n=3)	107	93-120
Heparin Plasma (n=3)	101	91-123
Serum (n=6)	102	91-108

Linearity

To assess the linearity of the assay, five or more samples containing or spiked with various concentrations of mouse IL-17 in each matrix were diluted with Calibrator Diluent and then assayed.

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