Human CCL5/RANTES Quantikine ELISA Kit

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Human CCL5/RANTES Quantikine ELISA Kit

Human CCL5/RANTES Quantikine ELISA Kit Summary

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货号：DRN00B

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)

Sensitivity

6.6 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)

Specificity

Natural and recombinant human RANTES

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human RANTES Immunoassay is a 3.5 hour solid phase ELISA designed to measure human RANTES levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human RANTES and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human RANTES showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human RANTES.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	91.9	573	1120	92	511	1105
Standard Deviation	2.3	10	32.4	6.2	32.7	70.7
CV%	2.5	1.7	2.9	6.7	6.4	6.4

EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	108	599	1191	88	494	1063
Standard Deviation	3.9	14.3	41.5	9.1	43.1	89.6
CV%	3.6	2.4	3.5	10.3	8.7	8.4

Recovery

The recovery of RANTES spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	95-110
Platelet-poor Citrate Plasma (n=4)	99	93-105
Platelet-poor EDTA Plasma (n=4)	98	93-103
Platelet-poor Heparin Plasma (n=4)	99	93-105
Serum (n=5)	101	94-107
Urine (n=10)	102	88-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RANTES were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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