Human GDF-15 Quantikine ELISA Kit

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Human GDF-15 Quantikine ELISA Kit

Human GDF-15 Quantikine ELISA Kit Summary

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货号：DGD150

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL), Urine (10 uL)

Sensitivity

4.39 pg/mL

Assay Range

23.4 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human GDF-15

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human GDF-15 immunoassay is a 3.5 hour solid phase ELISA designed to measure GDF-15 in cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human GDF-15 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural GDF-15 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of naturally occurring GDF-15.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	238	456	886	225	442	900
Standard Deviation	4.27	9.86	25	13.4	20.6	50.5
CV%	1.8	2.2	2.8	6	4.7	5.6

Recovery

The recovery of GDF-15 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	100	94-106
EDTA Plasma (n=4)	97	85-108
Heparin Plasma (n=4)	96	86-108
Serum (n=4)	98	88-107
Urine (n=4)	102	88-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of GDF-15 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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