Human Urinary TIM-1/KIM-1/HAVCR Quantiki

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Human Urinary TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

Human Urinary TIM-1/KIM-1/HAVCR Quantikine ELISA Kit Summary

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货号：DKM100

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Urine (50 uL)

Sensitivity

0.046 ng/mL

Assay Range

0.2 - 10 ng/mL (Urine)

Specificity

Natural and recombinant human TIM-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human TIM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIM-1 in urine. It contains NS0-expressed recombinant human TIM-1 and antibodies raised against the recombinant factor. Natural human TIM-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIM-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	0.98	3.04	5.88	1.09	3.19	6.23
Standard Deviation	0.042	0.119	0.259	0.069	0.193	0.484
CV%	4.3	3.9	4.4	6.3	6	7.8

Recovery

The recovery of TIM-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Urine (n=20)	104	94-112

Linearity

To assess the linearity of the assay, samples containing high concentrations of TIM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. For more information on linearity and hand

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