Human CD25/IL-2 R alpha Quantikine ELISA

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Human CD25/IL-2 R alpha Quantikine ELISA Kit

Human CD25/IL-2 R alpha Quantikine ELISA Kit Summary

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货号：DR2A00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL)

Sensitivity

10 pg/mL

Assay Range

78.0 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IL-2 sR alpha

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-2 sR alpha Immunoassay is a 3.5 hour solid phase ELISA designed to measure IL-2 sR alpha in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human IL-2 sR alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-2 sR alpha showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2 sR alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	207	613	2357	188	630	2615
Standard Deviation	12.7	37.5	108	13.6	38.1	183
CV%	6.1	6.1	4.6	7.2	6	7

Recovery

The recovery of IL-2 sR alpha spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	105	93-118
EDTA Plasma (n=5)	103	91-113
Heparin Plasma (n=5)	104	98-113
Serum (n=5)	105	99-116

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