Human Total IL-18/IL-1F4 Quantikine ELIS

返回产品中心

Human Total IL-18/IL-1F4 Quantikine ELISA Kit

Human Total IL-18/IL-1F4 Quantikine ELISA Kit Summary

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

货号：DL180

Assay Length

4.0 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL), Saliva (25 uL), Urine (50 uL)

Sensitivity

5.15 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)

Specificity

Natural and recombinant human Total IL-18.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Total IL-18/IL-1F4 Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IL-18 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IL-18 and has been shown to accurately quantitate the recombinant factor free and in complex with IL-18 BPa. Results obtained using natural human IL-18 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	175	301	636	197	381	739
Standard Deviation	5.49	8.06	24.6	27.4	46.7	81.1
CV%	3.1	2.7	3.9	13.9	12.3	11

Serum, EDTA Plasma, Heparin Plasma, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	126	251	495	126	248	494
Standard Deviation	3.85	6.33	14.6	10.9	19.7	41.6
CV%	3.1	2.5	2.9	8.7	7.9	8.4

Recovery

The recovery of human Total IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	96	90-102
EDTA Plasma (n=4)	104	93-113
Heparin Plasma (n=4)	102	88-110
Saliva (n=4)	108	94-120
Serum (n=4)	100	96-105
Urine (n=4)	91	84-100

Human CD25/IL-2 R alpha Quantikine ELISA Kit

Human FGF-21 Quantikine ELISA Kit

返回 产品中心