Human FGF-21 Quantikine ELISA Kit

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Human FGF-21 Quantikine ELISA Kit

Human FGF-21 Quantikine ELISA Kit Summary

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货号:DF2100

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

8.69 pg/mL

Assay Range

31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human FGF-21

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human FGF-21 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FGF-21 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human FGF-21 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FGF-21 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FGF-21.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	204	672	1324	245	742	1411
Standard Deviation	8	19.8	46.2	26.6	47.2	73.9
CV%	3.9	2.9	3.5	10.9	6.4	5.2

Recovery

The recovery of FGF-21 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	93-108
EDTA Plasma (n=4)	103	92-112
Heparin Plasma (n=4)	98	83-111
Serum (n=4)	105	87-119

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