Human ICAM-1/CD54 Allele-specific Quanti

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Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit

Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit Summary

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货号：DCD540

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

2 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.254 ng/mL

Assay Range

1.6 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human sICAM-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human sICAM-1 immunoassay is a 2.0 hour solid phase ELISA designed to measure sICAM-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human sICAM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring sICAM-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	4.61	12.1	19.6	4.77	12.5	20.5
Standard Deviation	0.17	0.63	0.98	0.32	0.67	0.9
CV%	3.7	5.2	5	6.7	5.4	4.4

Recovery

The recovery of sICAM-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	104	90-109

Linearity

To assess the linearity of the assay, samples spiked with or containing high concentrations of sICAM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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