Rat VEGF Quantikine ELISA Kit

Rat VEGF Quantikine ELISA Kit Summary

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货号：RRV00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

25 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant rat VEGF. This assay cross-reacts 59% with Mouse VEGF120 (E.Coli-expressed), 73% with Mouse VEGF164 (Sf21-expressed), 14% with Canine VEGF164 (E.Coli-expressed), 26% with Human VEGF121 (Sf21-expressed), 31% with Human VEGF121 (E.Coli-expressed), 24% with Mouse VEGF162 (NS0-expressed), 0.2% with Human VEGF206 (E.Coli-expressed) and 5.2% with Human VEGF/PIGF.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Rat VEGF Immunoassay is a 3.5 hour solid-phase ELISA designed to measure rat VEGF in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant rat VEGF and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat VEGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat VEGF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	71	65	71
Mean	103	191	891	107	233	900
Standard Deviation	3.8	10.7	19.7	8.5	23.3	41.1
CV%	3.7	5.6	2.2	7.9	10	4.6

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	65	60	65
Mean	79.4	174	797	87.6	204	828
Standard Deviation	5.7	7	14.1	8.2	21.2	42.5
CV%	7.2	4	1.8	9.4	10.4	5.1

Recovery

The recovery of rat VEGF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	103	94-108
EDTA Plasma (n=6)	102	95-108
Heparin Plasma (n=6)	92	80-100
Serum (n=6)	99	90-116

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of rat VEGF in each matrix were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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