BMP-2 Quantikine ELISA Kit

返回产品中心

BMP-2 Quantikine ELISA Kit

BMP-2 Quantikine ELISA Kit Summary

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

货号：DBP200

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Bone Extracts (50 uL)

Sensitivity

29 pg/mL

Assay Range

62.5 - 4,000 pg/mL (Cell Culture Supernates, Bone Extracts)

Specificity

Natural and mammalian-expressed recombinant human BMP-2. This assay does not recognize E. coli-expressed BMP-2.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human BMP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BMP-2 in bone tissue extracts and cell culture supernates. It contains CHO cell-expressed recombinant human BMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse BMP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BMP-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Bone Extracts

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	323	1010	2313	344	1043	2213
Standard Deviation	8.3	28.3	56	25.2	55.4	140.2
CV%	2.6	2.8	2.4	7.3	5.3	6.3

Recovery

The recovery of recombinant human BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
2 M Guanidine-HCI (n=1)	98	94-101
2 M Urea (n=1)	101	92-106
Cell Culture Media (n=4)	102	94-110

Linearity

The following samples were spiked with rhBMP-2 and then diluted with Calibrator Diluent. The samples were then serially diluted and tested to assess the linearity of the assay.

Rat VEGF Quantikine ELISA Kit

Human Total MMP-3 Quantikine ELISA Kit

返回 产品中心