Human Total MMP-3 Quantikine ELISA Kit

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Human Total MMP-3 Quantikine ELISA Kit

Human Total MMP-3 Quantikine ELISA Kit Summary

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货号：DMP300

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.045 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)

Specificity

Natural and recombinant human total MMP-3

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Total MMP-3 Immunoassay is a 4.5 hour solid phase immunoassay designed to measure total MMP-3 (pro- and active MMP-3) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Pro-MMP-3 and antibodies raised against the recombinant factor. Both antibodies also recognize recombinant human active MMP-3. Natural human MMP-3 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human MMP-3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	0.962	3.21	5.87	0.994	3.08	5.7
Standard Deviation	0.062	0.197	0.332	0.085	0.217	0.449
CV%	6.4	6.1	5.7	8.6	7	7.9

Recovery

The recovery of MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	96	87-104
Heparin Plasma (n=5)	92	85-106
Serum (n=5)	90	86-96

Linearity

To assess the linearity of the assay, samples containing high concentrations of MMP-3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Data Examples

BMP-2 Quantikine ELISA Kit

Human Thrombopoietin Quantikine ELISA Kit

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