Human Thrombopoietin Quantikine ELISA Ki

返回产品中心

Human Thrombopoietin Quantikine ELISA Kit

Human Thrombopoietin Quantikine ELISA Kit Summary

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

货号：DTP00B

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL)

Sensitivity

18.5 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Tpo

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Tpo Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Tpo in cell culture supernates, serum, and plasma. It contains recombinant human Tpo and antibodies raised against the recombinant factor. Results obtained using natural human Tpo showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural Tpo.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	194	512	983	187	517	970
Standard Deviation	17.5	16.5	26.8	16.3	39.2	63.9
CV%	9	3.2	2.7	8.7	7.6	6.6

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	204	579	1140	203	555	1070
Standard Deviation	13.7	15.1	52.6	19.3	48.7	92.8
CV%	6.7	2.6	4.6	9.5	8.8	8.7

Recovery

The recovery of Tpo spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	99	89-109
Platelet-poor EDTA Plasma (n=4)	95	85-102
Platelet-poor Heparin Plasma (n=4)	93	86-106
Serum (n=4)	102	90-111

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of Tpo were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Total MMP-3 Quantikine ELISA Kit

Human PDGF-BB Quantikine ELISA Kit Summary

返回 产品中心