Glucagon Quantikine ELISA Kit

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Glucagon Quantikine ELISA Kit

Glucagon Quantikine ELISA Kit Summary

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货号：DGCG0

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

14.7 pg/mL

Assay Range

31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural Glucagon. This assay cross-reacts <12% with Oxyntomodulin.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Glucagon Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Glucagon in cell culture supernates, serum, and plasma. It contains natural porcine Glucagon as the standard. The antibodies were raised against a human Glucagon synthetic peptide. This immunoassay has been shown to accurately quantitate human, mouse, rat, and porcine Glucagon.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	315	618	1024	354	653	1080
Standard Deviation	10.3	22	27.6	30.7	37.8	63.6
CV%	3.3	3.6	2.7	8.7	5.8	5.9

Recovery

The recovery of Glucagon spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	91-108
Human EDTA Plasma (n=4)	98	86-107
Human Heparin Plasma (n=4)	96	88-100
Human Serum (n=4)	102	95-111
Mouse Serum (n=2)	99	92-106
Porcine Serum (n=2)	97	91-107
Rat Serum (n=1)	93	86-101

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of Glucagon were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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