Mouse/Rat IL-22 Quantikine ELISA Kit

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Mouse/Rat IL-22 Quantikine ELISA Kit

Mouse/Rat IL-22 Quantikine ELISA Kit Summary

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货号：M2200

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

8.2 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse and rat IL-22

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse/Rat IL-22 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IL-22 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-22 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse or rat IL-22 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse and rat IL-22.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	35	35	35
Mean	45.9	129	628	43.9	122	596
Standard Deviation	1.9	7.5	22.1	3.4	6.7	25.2
CV%	4.2	5.8	3.5	7.7	5.5	4.2

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	34	34	34
Mean	44.4	117	692	45.4	128	662
Standard Deviation	2	7	25.7	4.8	7.5	35.5
CV%	4.4	6	3.7	10.5	5.9	5.4

Recovery

The recovery of IL-22 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Mouse Cell Culture Supernates (n=4)	98	93-103
Mouse EDTA Plasma (n=4)	95	86-106
Mouse Heparin Plasma (n=4)	94	83-102
Mouse Serum (n=4)	91	84-99
Rat Cell Culture Supernates (n=4)	98	91-102
Rat EDTA Plasma (n=4)	97	91-111
Rat Heparin Plasma (n=4)	93	88-101
Rat Serum (n=4)	96	90-107

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