Human Angiopoietin-1 Quantikine ELISA Ki

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Human Angiopoietin-1 Quantikine ELISA Kit

Human Angiopoietin-1 Quantikine ELISA Kit Summary

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货号：DANG10

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Platelet-poor Citrate Plasma (10 uL), Saliva (25 uL), Human Milk (25 uL)

Sensitivity

10.3 pg/mL

Assay Range

62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Saliva, Human Milk)

Specificity

Natural and recombinant human Angiopoietin-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Angiopoietin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Angiopoietin-1 in cell culture supernates, serum, plasma, saliva, and human milk. It contains NS0-expressed recombinant human Angiopoietin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Angiopoietin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Angiopoietin-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	611	1217	2502	619	1223	2496
Standard Deviation	20.8	24.2	67	42.4	71.5	113
CV%	3.4	2	2.7	6.8	5.8	4.5

Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	587	1179	2339	620	1230	2533
Standard Deviation	14	28.5	76.1	39.5	67.1	141
CV%	2.4	2.4	3.3	6.4	5.5	5.6

Recovery

The recovery of Angiopoietin-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	104	97-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Angiopoietin-1 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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