Human CCL4/MIP-1 beta Quantikine ELISA K

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Human CCL4/MIP-1 beta Quantikine ELISA Kit

Human CCL4/MIP-1 beta Quantikine ELISA Kit Summary

货号：DMB00

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.0 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 uL), Serum (150 uL), EDTA Plasma (150 uL), Heparin Plasma (150 uL), Citrate Plasma (150 uL)

Sensitivity

11 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates), 31.2 - 2,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human MIP-1 beta

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MIP-1 beta Immunoassay is a 3.0 or 4.5 hour solid phase ELISA designed to measure MIP-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-1 beta (Act-2 variant) and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant human MIP-1 beta. Results obtained using natural human MIP-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIP-1 beta.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	23.3	98.8	428	31.4	118	476
Standard Deviation	1.1	3.8	17.3	3.7	9.9	28
CV%	4.7	3.8	4	11.8	8.4	5.9

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	51.3	208	932	58.8	220	949
Standard Deviation	4.6	7.5	30.2	5.7	15.6	70.5
CV%	9	3.6	3.2	9.7	7.1	7.4

Recovery

The recovery of MIP-1 beta spiked to three levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	107	81-117
Citrate Plasma (n=5)	99	87-116
EDTA Plasma (n=5)	100	84-112
Heparin Plasma (n=5)	99	90-112
Serum (n=5)	99	88-113

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