Human VEGF-C Quantikine ELISA Kit

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Human VEGF-C Quantikine ELISA Kit

Human VEGF-C Quantikine ELISA Kit Summary

货号:DVEC00

规格：96T

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)

Sensitivity

48.4 pg/mL

Assay Range

109.0 - 7,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Plasma, Heparin Plasma, Saliva)

Specificity

Natural human VEGF-C, recombinant human VEGF-C (wild type), and recombinant human VEGF-C (Cys156Ser substituted)

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human VEGF-C Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VEGF-C in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human VEGF-C (Cys156Ser substituted) and has been shown to accurately quantitate the recombinant factor. Results obtained using recombinant human VEGF-C (wild type) and natural human VEGF-C showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VEGF-C.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	572	1622	3243	520	1527	3069
Standard Deviation	39.3	54.4	136	50.1	102	200
CV%	6.9	3.4	4.2	9.6	6.7	6.5

Serum, EDTA Plasma, Platelet-poor Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	512	1543	3195	514	1540	3066
Standard Deviation	33.8	54.2	136	43.6	110	198
CV%	6.6	3.5	4.3	8.5	7.2	6.4

Recovery

The recovery of VEGF-C spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	107	93-115
EDTA Plasma (n=4)	101	96-110
Heparin Plasma (n=4)	97	88-103
Serum (n=4)	99	89-106

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