Human Cytochrome c Quantikine ELISA Kit

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Human Cytochrome c Quantikine ELISA Kit

Human Cytochrome c Quantikine ELISA Kit Summary

Catalog # Availability Size / Price

DCTC0 1 Kit / Please Inquire
SCTC0 1 Pack (6 Plates) / Please Inquire

PDCTC0 1 Pack (50 Plates) / Please Inquire

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Lysates (20 uL), Subcellular Fractions (100 uL)

Sensitivity

0.31 ng/mL

Assay Range

0.6 - 20 ng/mL (Cell Lysates, Subcellular Fractions)

Specificity

Natural human Cytochrome c

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with other substances.

Product Summary

The Quantikine Human Cytochrome c Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human cytochrome c in cell lysates.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Lysates, Subcellular Fractions

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	3.64	7.31	10.7	3.54	6.74	10.2
Standard Deviation	0.15	0.3	0.42	0.18	0.34	0.51
CV%	4.1	4.1	3.9	5.1	5	5

Linearity

To assess the linearity of the assay, six cell lysates containing high concentrations of Cytochrome c were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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