Human Progranulin Quantikine ELISA Kit

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Human Progranulin Quantikine ELISA Kit

Human Progranulin Quantikine ELISA Kit Summary

货号：DPGRN0 规格：96T

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (25 uL), Human Milk (10 uL)

Sensitivity

0.54 ng/mL

Assay Range

1.6 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

Specificity

Natural and recombinant human Progranulin. Trypsin and Elastase cleaved Progranulin show reduced recognition in this assay, demonstrating specificity for Progranulin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Progranulin immunoassay is a 4.5 hour solid phase ELISA designed to measure Progranulin in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Progranulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Progranulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Progranulin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	13.5	32.7	64.8	14.4	33.1	64.4
Standard Deviation	0.7	1.9	4.1	1	2.3	4.4
CV%	5.2	5.8	6.3	6.9	6.9	6.8

Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	15.5	36.6	70.8	15.7	35.8	69.1
Standard Deviation	0.9	1.7	3.1	1.3	2.9	5.1
CV%	5.8	4.6	4.4	8.3	8.1	7.4

Recovery

The recovery of Progranulin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	107	103-112
EDTA Plasma (n=4)	102	95-110
Heparin Plasma (n=4)	100	92-108
Serum (n=4)	104	90-115
Urine (n=4)	109	99-116

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Progranulin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Product Summary

Precision

Recovery

Linearity

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