Porcine IFN-gamma Quantikine ELISA Kit

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Porcine IFN-gamma Quantikine ELISA Kit

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Porcine IFN-gamma Quantikine ELISA Kit Summary

货号：PIF00 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)

Sensitivity

11.2 pg/mL

Assay Range

39.0 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant porcine IFN-gamma

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Porcine IFN-gamma Immunoassay is a 4 hour solid-phase ELISA designed to measure porcine IFN-gamma in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant porcine IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring porcine IFN-gamma showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IFN-gamma.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	29	34	34
Mean	124	272	913	120	282	904
Standard Deviation	5.8	7.4	26.5	14	15.7	84.7
CV%	4.7	2.7	2.9	11.7	5.6	9.4

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	36	32	36
Mean	226	449	1518	227	515	1522
Standard Deviation	10.1	12	40.4	17.1	51.3	97.7
CV%	4.5	2.7	2.7	7.5	10	6.4

Recovery

The recovery of porcine IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	97	84-116
EDTA Plasma (n=5)	98	85-111
Heparin Plasma (n=5)	106	86-118
Serum (n=5)	98	81-119

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of porcine IFN-gamma in each matrix were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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