Mouse Chemerin Quantikine ELISA Kit

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Mouse Chemerin Quantikine ELISA Kit

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Mouse Chemerin Quantikine ELISA Kit Summary

货号：MCHM00 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

3.47 pg/mL

Assay Range

46.9 - 3,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse Chemerin

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse Chemerin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Chemerin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature Chemerin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Chemerin. Results obtained using natural mouse Chemerin showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Chemerin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	153	405	1124	168	385	1065
Standard Deviation	13	16.2	99	14.2	25.7	64.1
CV%	8.5	4	8.8	8.4	6.7	6

Recovery

The recovery of mouse Chemerin spiked to three levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=4)	102	89-117

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse Chemerin in each matrix were diluted with Calibrator Diluent and assayed.

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